Validation of an UHPLC-MS/MS Method for the Determination of Malachite Green, Leucomalachite Green, Crystal Violet, and Leucocrystal Violet in Shrimp, Fish, and Salmon Muscle Using a Modified QuEChERS Approach

A quantitative and confirmatory method for detecting the presence of triphenylmethane dyes in shrimp muscle using ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) a quick, easy, cheap, effective, rugged safe (QuEChERS) extraction approach was validated. The exhibited linearity selectivity coefficient determination (R2) higher than 0.95 all studied analytes. Limits detection (LODs) varied from 0.32 to 0.44 µg kg-1 limit quantification (LOQ) determined be 0.5 trueness, precision, decision limits (CCα), capability (CCβ) uncertainty presented adequate performance. In addition validation muscle, fish salmon were also satisfactory validated as an extension scope. suitability proposed evaluated through interlaboratory proficiency test, which results obtained. fully is thus suitable analysis shrimp, fish, muscle.